Question

Consider the case of in vitro evolution used to generate RNA molecules with ligase activity. If the researchers repeated the investigation using a higher fidelity DNA polymerase during PCR, the _______ would most likely be _______.
A. selection intensity; higherB. selection intensity; lowerC. population size; higherD. mutation rate; higherE. mutation rate; lower

Answer 1

Answer:

The answer is E.

Explanation:

In vivo or in vitro evolution (Directed Evolution) is a technique used by genetic scientists to push the change in nucleic acids or proteins in a specific direction to get the end results that they want.

And high-fidelity polymerase is used to get a replica of the target DNA that has less errors.

So the situation given in the question where researchers use a higher-fidelity DNA polymerase in vitro evolution, the mutation rate would most likely be lower because of the high-fidelity DNA polymerase.

I hope this answer helps.