Answer:
option A
Explanation:
In carrying out these experimental procedure, the same restriction enzyme that is used to cut out the gene of interest should also be the same used for the cutting of the plasmid to generate sticky ends. Then the desired gene is inserted into the cut plasmid then treatment with DNA ligase. Purified DNA ligase is utilized in gene cloning to join/ligate DNA molecules together forming a recombinant DNA. Thus, step 3 should be carried out after step 4.
d. coal because coal forms over millions of years, not just that, even now, our earth have resources
When the body perspires, it cools itself due to the high specific heat capacity of water. Perspiration produces a cooling effect.
The clinical manifestations should the nurse expect are reports of leg fatigue, tortuous veins in the legs and pain in lower extremities when standing.
In addition, leg fatigue is a communal clinical manifestation triggered by venous stasis and insufficient tissue oxygenation. The vein walls deteriorate and dilate causing distended, bulging veins that look tortuous and darkened. As vein walls weaken and dilate venous pressure increases and the valves turn out to be useless in which vein stasis and inadequate oxygenation end result is limb discomfort. The discolored toenails effect from a fungus below the nail or chronic hypoxia, not varicose veins, confined to a small area of heat in a calf is a mark of thrombophlebitis and reddened zones on a leg are symptomatic of thrombophlebitis.
Long answer short- to reproduce cells.
