Answer:
a. Bands at 1kb, 6kb and 8kb
Explanation:
The EcoRI and BamHI are the restriction enzymes which cut the DNA sequence especially a plasmid at specific sites called the restriction sites.
The restriction enzymes produces bands of specific length therefore these restriction enzymes are used to estimate the approximate length of the DNA.
In the given question, the
1. EcoRI- produces two strands of 7 kb and 8 kb
2. BamHI- produces two strands of 1kb and 14kb
This shows that the length of DNA sequence is 15kb
But when the DNA strand are digested with both the enzymes simultaneously then it will produce three bands as:
i) 14 kb can be broken down in 2 bands of 6 kb and 8 kb
ii) 1 kb band is already produced by the Bam HI.
This shows that 1+6+8= 15 kb
Thus, Option-A is correct.
S phase of interphase where the DNA replication takes place.
A good extraction solvent should have a strong solubilizing capability for the compound of interest, it should be immiscible or only weakly misciblewith the matrix solvent ( the first solution or mixture containing the compound from its natural source, e.g., water/ether. water/ chloroform, etc. If possible the extraction solvent should be non-flammable, non-toxic or of low toxicity, reasonably volatile, and of low eco-impact. Inexpensive and available, of high purity , and shelf stable. If one is determining the compound of interest by UV/Vis spectrophotometry or fluorescence, the solvent should have extremely low absobance or emission at the wavelength of analysis
• high solubility for the solute and low solubility for the carrier liquid.
• density difference vs. the carrier liquid greater than 150 kg/m. ...
• mid-level interfacial tension (5–30 dyne/cm)
• high resistance to thermal degradation.
