1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
schepotkina [342]
4 years ago
12

Targeted gene knockouts involve the deletion or disruption of a given gene. What method might be effectively used to knock out a

gene in yeast? What method might be used in mice? Why would the method used in yeast be ineffective in mice?
Biology
1 answer:
Vadim26 [7]4 years ago
7 0

Answer:

In order to determine the function of an unknown gene knockout technique is used. PCR based deletion strategy is used for gene knockout in the case of yeast, which is unicellular species and eukaryotic. On the other hand, in the case of a mouse, which is multicellular and eukaryotic, for gene knock out homologous recombination technique is used.  

The PCR based strategy is not appropriate for mice genes knockout, as mice are multicellular species and exhibit complex genome. While yeast constitutes some circular plasmids, known as 2 microns plasmids, which allows PCR based mutagenesis.  

You might be interested in
One polled (hornless) bull is bred to 3 cows. With Cow A, which is horned, a polled calf is produced. With Cow B, also horned, a
Arisa [49]

Answer:

Bull = Pp

Cow A = pp

Cow B = pp

Cow C = Pp

Explanation:

In cows polled (P) is dominant over horned (p) trait.

The bull is polled so it can be PP or Pp. Cow B is horned so it must be pp. Resultant offspring from bull X Cow B is also a horned calf (pp). This calf must have received one p from the bull. So the bull's genotype is Pp.

Cow A is also a horned cow so it must be pp. When bull X Cow A, a polled calf is produced. Since the bull's genotype is Pp and Cow A's genotype is pp, this polled calf will be Pp like the bull.

Cow C is polled like the bull so it can be PP or Pp. However on crossing with the bull (Pp) a horned calf is produced (pp). This calf got one p allele from the bull and p allele from the Cow C. So Cow C's genotype must be Pp.

Hence,

Bull = Pp

Cow A = pp

Cow B = pp

Cow C = Pp

5 0
3 years ago
When elements that form a mineral dissolve in hot water, they form a mixture called a(n) ______
ryzh [129]

Answer:

I believe it's G. Solution

Explanation:

Hope my answer has helped you!

3 0
3 years ago
Read 2 more answers
Do all planets move in the same way and the same speed
mylen [45]

Answer:

no

the speed is affected by the mass of the planets and venus moves in the opposite direction to the other plants

8 0
3 years ago
What happens to co2 levels when plants are in the dark and when they are exposed to light?
r-ruslan [8.4K]
Co2 levels stay the same then drop when there's sunlight.<span />
5 0
4 years ago
Why do gardeners add peat moss to soil
Temka [501]
Peat moss allows a soil a greater ability to not just hold water, but to hold it in a way that it's available to plant roots. If a soil is too clay, it will hold water but not give it up. If a soil is too sandy, water drains away too quickly.
4 0
4 years ago
Other questions:
  • Which molecule carries the energy produced during cellular respiration?
    8·2 answers
  • The ecological problem that resulted from Ddt Use is called
    6·2 answers
  • One gene determines red vs. white flowers in pea plants, and another determines axial vs. terminal flowers. Two true-breeding st
    5·2 answers
  • Which of the following is a group of therapsids
    9·2 answers
  • A woman's prepregnant weight is within the normal range. during her second trimester, the nurse would determine that the woman i
    13·1 answer
  • 10. A UTI is a common urinary system disorder that's typically treated with A. blood transfusions. B. coagulants. C. antibiotics
    12·1 answer
  • Biological molecules used by living systems to catalyze specific reactions
    13·1 answer
  • CAN SOMEONE HELP ME PLSS!!!!!!!!!!!!!!!! This is due today. It doesn't have to be super long at least 7 sentences. Explain how t
    6·1 answer
  • If two parents of the color are bred together, is there a chance that their offspring can be born with a different fur color? Ex
    5·1 answer
  • Which of the follwoing is not a characteristic of life?aGrow and DevelopbContain DNAcSleep/RestdRespond to Stimuli
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!