In order to determine the function of an unknown gene knockout technique is used. PCR based deletion strategy is used for gene knockout in the case of yeast, which is unicellular species and eukaryotic. On the other hand, in the case of a mouse, which is multicellular and eukaryotic, for gene knock out homologous recombination technique is used.
The PCR based strategy is not appropriate for mice genes knockout, as mice are multicellular species and exhibit complex genome. While yeast constitutes some circular plasmids, known as 2 microns plasmids, which allows PCR based mutagenesis.
If a person skipped lunch is could cause a low blood sugar
level and this will likely affect the liver cells in having it to react in
which glycogenolysis occurs. This process in the liver cells is a way of
breaking down glycogen in a way to fuel the body as it turns into glucose.
