Question

The FRAP technique occurs in a series of steps. Select every statement that correctly describes a step in the FRAP procedure.A. The speed of repair of the fluorescent marker is measured.B. All fluorescent molecules in the cell are irreversibly bleached.C. The relative mobility of the fluorescently labeled molecule is measured.D. The molecule of interest is fluorescently labeled.

Answer 1

Answer:

The answer is "Option C and Option D"

Explanation:

FRAP is used only for protein motion management.  It travels throughout two dimensions in the plasma membrane. It includes many available-moving or immobile proteins.  This technology uses globally marked fluorescent bleaching products, and also includes the fluorescent proteins, but one particular area is washed.  

Its protein arc changes are detected by fluorescence reappearance. The fluorescence reappears at a certain stage if the protein can move freely. When the proteins aren't able to move quickly, fluorescent dyes are recovered after a little period, and fluorescence would not resurface during that point if the enzyme is immobile.