Answer:
The order must be K2→K1, since the permanently active K1 allele (K1a) is able to propagate the signal onward even when its upstream activator K2 is inactive (K2i). The reverse order would have resulted in a failure to signal (K1a→K2i), since the permanently active K1a kinase would be attempting to activate a dead K2i kinase.
Explanation:
- You characterize a double mutant cell that contains K2 with type I mutation and K1 with type II
mutation.
- You observe that the response is seen even when no extracellular signal is provided.
- In the normal pathway, i f K1 activat es K2, we expect t his combinat ion of two m utants to show no response with or without ext racell ular signal. This is because no matt er how active K1 i s, it would be unable to act ivate a mutant K2 that i s an activit y defi cient. If we reverse the order, K2 activating K1, the above observati on is valid. Therefore, in the normal signaling pathway, K2 activates K1.
Answer:
proteins; purify; expression
Explanation:
One of the products of recombinant DNA technology is antibiotics. To have penicillin, we used to have to breed fungus that produces the drugs. With recombinant DNA technology, you can extract the DNA that needed to produce penicillin, then put it into a bacteria using an expression vector. This technology will allow us to produce a specific protein (whether its enzyme, hormone, or anything) easier.
Occluded front I think this answer is correct
I'm working on the assignment rn. But here are some clues to help you.
Water is more of a radical it brings moisture and it has great power in its surf clouds are made from the moisture water is like a power sorce for storms and other things