ddNTPs have a hydrogen at 3' position which terminates synthesis of DNA.
ddNTPs is the abbreviated form for dideoxynucleotides triphosphates. They are known as chain elongating inhibitors. The Sanger method is a sequencing technique that is used to increase the number of DNA copies. The DNA synthesis requires magnesium ions, a single-stranded DNA template, oligonucleotide primer, and nucleotide precursors like dATP, dGTP, dTTP, and dCTP. Also, DNA polymerase is required which is an essential component for DNA synthesis.
The Sanger sequencing method is a significant process evident by its use in Human Genome Project which helped scientists in sequencing the human genome. The small sequenced fragments are joined together to form large regions subsequently forming chromosomes.
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Each atom becomes different from the next by having different number combinations of these subatomic particles. The different numbers of protons electrons and neutrons determine different elements or in other words a pure substance made from a solitary kind of atom
Every element has different qualities or physical characteristics for example oxygen and gold The former is a gas that is one of many elements making up organic matter Where as the latter is solid and inert a non-reactionary element
<span>C. The transferred electron from lithium to fluorine provides each atom with a full outer energy level.</span>
<span>the interference of two waves of equal frequency and opposite phase, resulting in their cancellation where the negative displacement of one always coincides with the positive displacement of the other.</span>
