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melamori03 [73]
4 years ago
7

Explain the process of DNA isolation.

Biology
1 answer:
Nutka1998 [239]4 years ago
7 0

Answer:

Lysis, precipitation and purification

Explanation:

DNA isolation is a molecular biology technique based on the extraction of DNA and subsequent purification to remove inhibitors that may hamper the process of amplification. DNA isolation consists of three steps:

1- Lysis: it consists of the mechanical disruption of the cell, where the nucleus is broken to release DNA. During this process, it is required to use enzymes and detergents such as Proteinase K

2- Precipitation: during this process, the DNA is precipitated by using alcohol and salt.  

3- Purification: this process removes the impurities from DNA. Extraction is carried out by washing DNA with ethanol and water.

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