Question

How could you compare the original plasmid and the engineered plasmid in the lab using restriction digestion and gel electrophoresis.

Answer 1

A common practice is to insert a gene of bioluminescence into the bacteria's plasmid so that identifying them becomes easy. However, if this method was not employed, then we can put the bacteria into growth cultures and give them conditions to promote the production of the protein coded by the inserted gene. The bacteria that produce the protein contain the engineered plasmids and those that do not contain the original plasmids.