Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
Answer:
d. ATP; Fructose-2,6-bisphosphate
Explanation:
Phosphofructokinase-1 is the enzyme that catalyzes the formation of fructose-1,6-bisphosphate from fructose-6-phosphate and ATP. The phosphofructokinase step is the first rate-limiting step of glycolysis.
Phosphofructokinase-1 activity is allosterically regulated. Its activity is increased whenever the cell's ATP supply is depleted or when its breakdown products, ADP and AMP accuulates in he cell. However, it is inhibited when the cell is amply supplied with ATP.
The activity of phosphofructokinase-1 is restored by fructose-2,6-bisphosphate, its most potent activator.
From the given options:
a. AMP :::: citrate is wrong because AMP increases the activity of phosphofructokinase-1 and citrate is not its activator but an inhibitor
b. AMP :::: Fru-2,6-P2 is wrong because AMP increases its activity same as fructose-2,6-bisphosphate
c. ATP :::: citrate is wrong because both citrate and ATP are inhibitors
d. ATP :::: Fru-2,6-P2 is correct as explained above
e. All of these is wrong because of the other wrong options above.
If you use soymilk on a regular basis, you should select a variety that's enriched with calcium and vitamins. The correct option in regards to the given question is option "D". Soymilk is actually a milk that is made from plant products. The soybeans are soaked in water and then they are grinded with water to produce the soymilk. Soymilk is thought to have originated in the country of China during the reign of the Han dynasty. It is a favorite of the people of east Asia and so used in numerous foods. Soymilk was first introduced in United States of America in the year 1979.
Answer:
B. Ethanol
Explanation:
During absence of O2 pyruvic acid is reduced to ethanol in alcoholic fermentation.
