The process by which living things produce more organisms like themselves is the reproduction. There are two types of reproduction namely; asexual reproduction where only one parent is involved, and sexual reproduction that needs two parents, such that the cells of the two parents (egg and sperm) join, resulting to a unique offspring since they have characteristics from each parent are passed down/combined.
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In in vitro plant tissue culture, indolbutyric acid and other auxins are used to initiate root formation in a procedure called micropropagation. The micropropagation of plants is an asexual propagation or propagation technique that is based on the organogenetic potential of plant cells, which consists of cultivating in vitro on appropriate substrates, isolated cells, portions of yolk meristems, vegetative apices at the beginning of their development or microstaquillas. Small samples of plants used are called explants. Auxins such as indolbutyric acid can be used to cause mass formation of undifferentiated cells called corns. Callus formation is often used as a first step in the micropropagation process since, by exposure to certain auxin hormones, callus cells can be induced to form other tissues such as roots.
Indolbutyric acid is often used to promote the rooting of stakes. In a study in Camellia sinensis, the effect of three different auxins, indolbutyric acid, indolacetic acid and 1-naphthalenacetic acid on root formation was measured. According to the authors, indolbutyric acid produced a higher root yield compared to other auxins.9 This effect of indolbutyric acid is consistent with that found in other studies; This hormone is considered the most commonly used auxin for root formation, 10 because it is much more potent than indolacetic acid and other synthetic auxins.7
Jatropha curcas is a species in which there is an improvement in the quality of the rooting of the stakes with the addition of indolbutyric acid in the middle (longer root length, number of roots, percentage of rooted stakes, and dry root weight) , 11 although not always the effect on rooting is statistically significant.
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Given that a colorimeter is an instrument used for chemical analysis by comparing a liquid’s color with standard colors. In an experiment, a scientist used two calorimeters and noted the readings. The first colorimeter showed consistent readings that were five points lower than the actual reading. The second colorimeter provided readings that were the same as the actual reading.
Though consistent, the first colorimeter has a zero error of -5.
Hence whenever first calorimeter is used this adjustment to be made i.e. actual reading +5 should be made to get correct reading
But second can be used as such taking the reading as correct.
