Question

You start a PCR with 1,000 molecules of DNA, an excess of primers and an excess of nucleotides. The PCR works perfectly and each round of PCR doubles the amount of DNA present. How many molecules of DNA would be present after 11 rounds of PCR?

Answer 1

Answer:

2.048 x 10^6 No. Of Copies

Explanation:

The DNA replication in a PCR works exponentially by using the 2^n where n represents the number of cycles.

For one molecule of DNA = 2^11 = 2048 No. Of Copies

For 1000 Molecules of DNA = 1000 x 2048 = 2.048 x 10^6 No. Of Copies