Answer:
On the exterior of the cell.
Explanation:
The black spots as mentioned in the given case would appear on the exterior of the cell after three hours, which suggests that the proteins or the enzymes had been discharged from the pancreatic cell. The mentioned method of labeling and then chasing the component is known as a pulse-chase experiment.
In this experiment, the labeled compounds are used to follow the dynamics of cellular pathways and procedures. The molecules in a cell get produced and degraded spontaneously at various rates. These changes in the localization of the molecule and its expression levels with time can be determined by exposing or pulsing cells to a labeled compound.
After this the cells are exposed sequentially to the same compound unlabeled, the process is termed as the chase. The compounds are generally labeled with fluorescent dyes or radioisotopes.
Answer:
Explanation:
Normally, under anaerobic condition in yeast, pyruvate produced from glycolysis leads to the production of ethanol as shown below.
pyruvate ⇒ acetaldehyde + NADH ⇒ ethanol + NAD
The pyruvate is converted to acetaldehyde by the enzyme, pyruvate decarboxylase. It should be NOTED that carbon dioxide is released in this step. The acetaldehyde produced in the "first step" is then converted to ethanol by the enzyme alcohol dehydrogenase. It must be noted from the above that the steps are irreversible.
If a mutated strain of yeast is unique because it does not produce alcohol and lactic acid (which is referred to as toxic acid in the question); thus having a high level of pyruvate because of the presence of a novel enzyme. <u>The function of this novel enzyme will most likely be the conversion of acetaldehyde in the presence of carbondioxide back to pyruvate; thus making that step reversible</u>. This could be a possible explanation for the high level of pyruvate present in the yeast.
Answer:
Progenies of batch I would not be labeled by 35S; progenies of batch II would be labeled by 32P.
Explanation:
Catabolism/destructive metabolism. Both terms are used to describe the process of breaking down complex molecules for its stored energy.
