Movement of lithospheric plates created isolation between populations and with isolation comes different mutations and lineages which is how evolution occurs. (This is called allopatric speciation!).
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
D. Applying fertilizer granules to your yard
· Abiotic factors are nonliving, such as temperature, sunlight, and soil in an ecosystem.
· Biotic factors are the living parts of an ecosystem, such as plants and animals.
Given the above, the biotic factor listed is evergreen trees in a coniferous forest.
The other options - soil, precipitation, and temperature - are abiotic.
Answer:
<span>C. evergreen trees present in a coniferous forest
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