The answer is burning coal! Please forgive me if I get this answer wrong!
Answer:
I think C.............................
Answer: A double restriction digest
Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no
Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.
This can happen because you have recessive and dominant characteristics passed down. So you can get a totally different chromosome that doesn't come from your parents because your grandparents may have had it or great grand parents, the line keeps going .