Sandwich Generation
This phenomenon is most prevalent in the developed world where the expectancy rates are high. Children are usually considered as 'Capital Intensive' while the elderly parents are considered as labor and time intensive hence balancing the needs by sandwiched parents is typically challenging.
It eats away into personal time and therefore increases one's risk of developing anxiety and depression.
The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
The question has incorrect option, however the question can be answered by the general explanation. On the basis of the general explanation the answer is as follows:
Answer:
The correct answer is - Serine has a small side chain that can fit in any relatively polar environment.
Explanation:
Serine is an amino acid that have a small side chain in comparison of other amino acid and can be replaced by another amino acid with very least effect on the structure and function of a particular protein as they can fit in any relatively polar environment.
Serine have hydroxyl group in their side chain. It is polar amino acid that can easily establish itself in any polar condition and cause least changes in protein structure and function.
Thus, the correct answer is - Serine has a small side chain that can fit in any relatively polar environment.