Answer:Chromatography is actually a way of separating out a mixture of chemicals, which are in liquid or gas form, by letting them flow slowly past another substance, which is either a solid or a liquid. It consist of a stationary phase and a mobile phase.
Explanation: All chromatographic systems rely on the fact that a substance placed in contact with two unmixable phases, one movable phase and one stationary phase, will equilibrate between them. A selectivity (or separation) factor (α) is used to 'chemically' distinguish between sample components. It is usually measured as a ratio of the retention (capacity) factors (k) of the two peaks in question and can be visualized as the distance between the apices of the two peaks. reproducible fraction will partition into each phase, depending on the relative affinity of the substance for each phase. A substance which has affinity for the moving or mobile phase will be moved rapidly through the system. A material which has a stronger affinity for the stationary phase, on the other hand, will spend more time immobilized in that phase, and will take a longer time to pass through the system. Therefore, it will be separated from the first substance. By definition, chromatography is a separation technique in which a sample is equilibrated between a mobile and a stationary phase. A theoritical plate or tray is used to produces the best possible difference between the liquid and vapour phases in equilibrium with it
Chromatographic separations are best done with a small amount of analyte (substance to be separated during analysis), which keeps either phase from becoming saturated with analyte, so that the concentrations in the two phases are directly proportional. Overloading the column with sample causes one of the phases to become saturated, leading to a loss of column efficiency, and poorly shaped peak profiles.
The retention volume in chromatographic separation (Vr) is the volume of the mobile phase required to carry the solute through the column to elution, is related to the column flow (Fc) and the retention time (tr). Likewise, the volume of the mobile phase(Vm), is related to the flow and the time the void volume takes to pass through the column.
Band broadening using the kinetic model is a phenomenon that reduces the efficiency of the separation being carried out, leading to poor resolution and chromatographic performance. This is problematical in terms of both the quality of the separation obtained and the accuracy with which sample components can be quantified.The wider band results in a dilution effect that produces a decrease in peak height accompanied by a loss in sensitivity and resolution. The eddy dispersion, accounts for the source of band broadening related to any flow unevenness in the column.
Naturalists represent the idea that only natural laws (physical laws) are the rules that govern the structure and behavior of the natural universe. This means that everything can be explained in terms of natural causes. Naturalism is the belief that all that exists material matter, that there is no such thing as soul and that individuals have no purpose in life.
<span>Guarana i think but i'm not very sure</span>
Answer:
If an inhibitory synapse fires at the same time and at the same distance from the initial segment as an excitatory synapse of the same intensity there will be no changes in the potential in the firing zone.
Explanation:
Under normal conditions, the transmembrane potential depends on the ionic charges present in the intracellular and extracellular spaces. The extracellular space load is usually positive and in the cytoplasm is negative.
- <u>Depolarization</u> occurs by opening ion channels that allow sodium to enter the cell, making the intracellular space more positive.
- An opening of potassium channels releases this ion to the extracellular space, leading to <u>hyperpolarization</u>.
An excitatory synapse is one capable of depolarizing a cell and boosting the production of action potential, provided it is capable of reaching the threshold of said potential.
On the other hand, an inhibitory synapse is able to hyperpolarize the cell membrane and prevent an action potential from originating, so that they can inhibit the action of an excitatory synapse.
The interaction between two synapses, one excitatory and one inhibitory, -called synapse summation- will depend on the strength that each of them possesses. In this case, the intensity of both synapses being the same, there will be no changes in the membrane potential in the firing zone.
Learn more:
Excitatory and inhibitory postsynaptic potentials brainly.com/question/3521553
