The answer is DNA ends. These ends often overhangs and anneal with another DNA end, forming a new base form. These ends will anneal with the use of hydrogen bonding which can help stabilize the ends. However, certain prerequisites must be followed to maintain the bond.
The unbonded terminals of the DNA are known as sticky ends. They are called sticky as they exhibit complementary bases. The generally used restriction enzymes cleave the two complementary strands of DNA at distinct locations, producing sticky ends or overhangs.
These sticky ends permit for perfect base pairing and it takes place due to hydrogen bonding. These sticky ends permit one to insert DNA. These sticky ends play an essential role in molecular cloning as they make sure that the fragment of DNA is administered into the plasmid in an accurate direction.
