The answer is B- 43 degrees North.
Calhoun was vice president of the United States in 1824 under John Quincy Adams and was re-elected in 1828 under Andrew Jackson. Jackson was for the Tariff of 1828 and caused Calhoun to be opposed to Jackson, which led to Calhoun's resignation in 1832.
I need more information or I can’t get the answer like is that from a textbook or something
I am not completely sure what this is asking but I believe it would be. That the evidence would be that the fish would be getting less oxygen hence causing noticeable breathing problems (probably best spotted by looking at the lungs).