- D
- A
- A,C,and F
<h2>Hope this helps!</h2>
Given what we know, after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
<h3>Why would this be the next step?</h3>
Once you have removed the loopful of broth culture from the tube, you should apply a flame to the end of the tube, this is of vital importance. The reason for this is to deny any other contaminants from entering or exiting the culture sample.
Therefore, we can confirm that after you’ve removed a loopful of broth culture from the culture tube you should immediately apply a flame to the open end of the test tube.
To learn more about culture tubes visit:
brainly.com/question/5228823?referrer=searchResults
Answer:
Because older cultures of gram-positive bacteria tend to lose their ability to retain crystal-violet in the peptidoglycan of their cell walls and can be confused with gram-negative bacteria.
Explanation:
Gram staining is used to differentiate between two major groups of bacteria. Gram-positive and gram-negative, these bacteria differ in the amount of peptidoglycan in their cell walls. Gram-positive bacteria have a higher amount of peptidoglycan, which absorbs the violet crystal complex used in gram staining, staining them purple/violet. Old cultures of gram-positive bacteria tend to lose the ability to retain the violet crystal and are stained by safranine, staining them red/pink and appear to be gram-negative.
<span>The need for a polymerase that wouldn't denature at high temperature. Obviously, organisms that live in hot springs and the like will have adapted enzymes that can survive at these temperatures.</span>
