Answer:
The gene of interest from another source.
Explanation:
A plasmid is a small, circular double-stranded extrachromosomal DNA molecule within a cell particularly a bacteria cell that is physically separated from chromosomal DNA and can also replicate independently.
Expression vector is usually a plasmid (or a virus can also be utilized) constructed to allow for gene expression in cells. The vector is used to introduce a gene of interest into a target cell, and can drive the cell's mechanism for protein synthesis to produce the protein encoded by the gene, thus giving rise to the expression of the gene.
This gene of interest is integrated into the plasmid that is cut by endonucleases and then ligated using DNA ligase. Thus the expression differs from the normal plasmid in that it contains an external gene sequence.
Answer:
Loyalty is the most overvalued value. ... When people demand your loyalty what they're usually trying to do is escape accountability. I am not saying loyalty has no value but rather being loyal to people and organizations who do not keep their promises, maintain standards, play favorites or exploit you is self-destructiv
Answer: After mitiosis, each daughter cell will also have 12 chromosomes. Cytokinesis is just the last stage of telophase, where the cell completely forms the two daughter cells. Telophase does not form chromosomes.
Explanation:
As of now Sudbury has some of the cleanest air in all of Ontario. That’s hard to believe given the city once emitted 2.5 million tonnes of sulfur dioxide per year due to the Lumber industry. In the 1980s, the “Sudbury” became known as a unit of pollution, against which other industrial cities were measured. It’s now become known as a unit of restoration.
