A plasmid is a small, circular double-stranded extrachromosomal DNA molecule within a cell particularly a bacteria cell that is physically separated from chromosomal DNA and can also replicate independently.
Expression vector is usually a plasmid (or a virus can also be utilized) constructed to allow for gene expression in cells. The vector is used to introduce a gene of interest into a target cell, and can drive the cell's mechanism for protein synthesis to produce the protein encoded by the gene, thus giving rise to the expression of the gene.
This gene of interest is integrated into the plasmid that is cut by endonucleases and then ligated using DNA ligase. Thus the expression differs from the normal plasmid in that it contains an external gene sequence.
The asnswer is 2DG also known as 2-Deoxy-D-glucose. 2DG is a glucose molecule that has the
2-hydroxyl group replaced by hydrogen, so that it cannot undergo further
glycolysis. As such; it acts to competitively inhibit the production of
glucose-6-phosphate from glucose at the phosphoglucoisomerase level (step 2 of
glycolysis).
Reasoning - Usually in an island surrounded by water has little of life because the limited resources but also if there were to be species living within the island it will be very little because of the resources they compete. Especially moving away from mainland since (No Humans) lol.
