How is mitochondrial DNA (mtDNA) typing used in forensic science? An unknown mtDNA sample is matched to a sample of nuclear DNA
2 answers:
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Answer:
A. Yes, we will see growth. Yes, they will glow.
Explanation:
pGLO plasmid was engineered to be used as a vector in the field of biotechnology. It has an ampicillin resistance gene which acts as a reporter gene. It also has another reporter gene, GFP which shares its promoter (araC) with the gene responsible for metabolizing arabinose. GFP gene is thus translated only in the presence of arabinose in the medium since the promoter is active only in the presence of arabinose. Once the GFP gene is translated, the colonies glow with green fluorescence under UV light.
Here, the <em>E.Coli</em> colonies were grown successfully on LB + Ampicillin + Arabinose plate so they were ampicillin resistant. They also glowed in UV light. If these transformed colonies are inoculated in another LB + Ampicillin + Arabinose plate they will be able to grow successfully since they are ampicillin resistant. They will also glow because the new plate also has arabinose so the araC promoter will be active and expression of both GFP gene and arabinose metabolizing gene will occur.
