Answer:
b. False
Explanation:
These components are not sufficient to successfully perform a PCR reaction. Deoxynucleoside triphosphates (dATP, dGTP, dCTP, dTTP) are missing so that the PCR reaction can occur perfectly.
The PCR technique allows a specific fragment of the DNA molecule to be amplified thousands of times in just a few hours. This technique revolutionized research in molecular biology because it had taken a long time for DNA amplification. From PCR it is possible to obtain enough copies of a part of DNA to detect and analyze the sequence that is the target of the study.
For a PCR reaction to be performed a solution with some components must be prepared. These components are:
- Magnesium chloride buffer (to optimize reaction and act as a cofactor for polymerase)
- Forward and reverse primers (to customize the start of the enzyme reaction)
- DNA polymerase (Enzyme required for replication of desired DNA region.)
- DNA Template (the DNA to be copied)
- PCR-grade water
- Deoxynucleoside triphosphates: dATP, dGTP, dCTP, dTTP (act as bricks in the construction of DNA molecules).
sunlight and nutrient availability
Lets say that the genotypes are:
Male affected:XbY
Male unaffected: XaY
Female affected: XbXb
Female carrier: XaXb
Female unaffected:XaXa
P: XbY x XaXa
F1: XaXb XaXb XaY XaY The middle square is female carrier.
P:XbY x XaXb
F1: XaXb XbXb XaY XbY The left square is affected female.
P:XaY x XbXb
F1: XaXb XaXb XbY XbY The right square is affected male.
We know that frequency of recombination is proportional with the distance between the genes on the chromosome. Therefore when the recombination rate is higher that means the distance between the genes on the chromosome is bigger. If the recombination rate is lower that means the genes are closer to each other on the chromosome. In this case the cross over rate is half the normal rate in the wild-type. That means that on the genetic map the distance between the two genes on the wild type will be twice bigger than the genes of the mutated Drosophila.
