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vodomira [7]
3 years ago
15

What is the most appropriate method to prepare a sample of cheek cells for PCR?

Biology
1 answer:
Dmitry [639]3 years ago
5 0

Answer:

Extraction of genomic DNA

Explanation:

Resuspend the cells(any number), smaller numbers also work, in 10-20 ul of TE buffer or milli Q water. boil for 5-10 min in a waterbath, quick chill, short spin. use the supernatant as a template for PCR.

If cells are very less, u can directly add cells to the PCR reaction without enzyme,boil for 5-10 min in a waterbath, let it cool slowly, short spin. Add enzyme to the supernatant and start the reaction at extension step followed by normal PCR reaction steps.

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The human eye can detect wavelengths between 400 nm and 700 nm. The sky appears blue (490 nm) due to the scattering of visible l
kondor19780726 [428]

Answer:

In the electromagnetic spectrum, the visible spectrum is the part, which is perceptible to the human eye. The electromagnetic spectrum in this array of wavelengths is known as simply light or the visible light. A general human eye will react towards the wavelengths in the range between 380 to 740 nm. A human eye sees the sky as blue that comes in the wavelength 490 nm because of the scattering of the visible light by the particles in the atmosphere. This concludes that 490 nm is the sole wavelength, which is reaching the eye.

3 0
3 years ago
Of the following muscle types, which has the longest muscle cells and has obvious stripes called striations? Of the following mu
kumpel [21]

Answer:

Explanation: skeletal muscle

6 0
3 years ago
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Carlo is wondering what causes plants to grow at different rates. Carlo's hypothesis is "Plants will grow more when the day is l
Sphinxa [80]

Options for the question have not been given. They are as follows:

type of soil

type of plants

exposure to sunlight

method for measuring the growth

Answer:

exposure to sunlight

Explanation:

A scientific experiment has an experimental group and a control group. Control group is included to omit any error in results due to the factors other than the variable factor. Experimental group is the one exposed to different levels of the variable factor.

Here, Carlo wants to determine the the effect of day length on plants' growth. The test variable here is thus the duration of sunlight. In experimental group, this duration will be changed to determine its effect. In control group it will be kept constant so that results from other factors can be excluded from the observed experimental result.

8 0
3 years ago
Albinism is inherited as autosomal recessive trait. The frequency of the disease in the population equals to 1:20 000. Calculate
prisoha [69]

Answer:

0.0139- frequency of heterozygotes in the population

Explanation:

Let q to the power of 2 represents the frequency of the homozygous recessive (aa) = 1/20000 = 0.00005

This, q = √q^2 = √0.00005 = 0.007

Since p + q = 1

p = 1 - 0.007 = 0.993

Using the formula: p^2 + 2pq + q^2

Where 2pq represents the frequency of the heterozygotes, thing we have

2 x 0.007 x 0.993

= 0.0139

8 0
3 years ago
You arrive at the scene of an accident where people are panicked and yelling. Three people appear to be injured. What action wil
Nezavi [6.7K]

Answer:

Go over to the panicked people and ask what happened.

Explanation:

I hope this helps you!

7 0
3 years ago
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