This is because there might not be one restriction site bordering the gene to be cloned and the identical restriction site in the plasmid. Since these two restriction enzymes make compatible sticky ends, the insert has a chance of combining with the plasmid. To ensure efficient digestion, the two recognition sites should be more than 10 base pairs apart. If one of the enzymes is a poor cutter or if the sites are separated 10 base pairs or less, the digestions <span>should be performed sequentially</span>
Answer:
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Explanation: answer in the comment
The most specific category and level shared by humans, gorillas, and howler monkeys (from the new world) are Suborder Anthropoidea.
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What is suborder Anthropoidea?</h3>
Anthropoidea is a suborder of primates that includes humans, gorillas, and monkeys. Anthropoids are larger than prosimian primates and have flatter, more human-like faces. They also have larger brains.
Unquestionably apes, dryopithecines lived in the following Miocene. According to the conventional palaeontological theory, these Miocene apes gave rise to three new lineages in turn, one of which led to gibbons, another to big apes, and the third to humans.
However, it has long been held that the ancestors of both gibbons and orang-utans diverged from the ancestral line of the advanced Primates at an early date and that this line only later split into two groups, one of which included humans and the other of which included the gorilla and the chimpanzee; recent palaeontological evidence now tends to support this second view.
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Answer: Carbon dioxide, water, and light energy
Explanation: