Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
The answer is actually <u>true</u><u>.</u>
The starting point of all rivers is higher than their end point. However, under the right conditions, small amounts of water can be drawn upwards, against the tug of gravity, through a phenomenon known as "capillary action". For this to occur, however, the water must be confined into a small flow space.
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Answer:
Environmental Sciences is a multidisciplinary academic field that integrates biological, physical and information sciences for the study of the environment and solutions to environmental problems. These sciences include physics, ecology, zoology, biology, chemistry, mineralogy, oceanography, geology, soil sciences, atmosphere sciences and geography. The environmental sciences emerged from the fields of natural history and medicine. Today, they allow an integrated, quantitative and interdisciplinary approach to the study of environmental systems.
Related areas of study include environmental studies and environmental engineering. Environmental studies integrate social sciences to understand human relations, perceptions and policies regarding the environment. Environmental engineering focuses on design and technology designed to improve the quality of the environment. Environmental problems almost always include the interaction of physical, chemical and biological processes. Environmental scientists work on topics such as understanding ground processes, assessing alternative energy systems, controlling and mitigating pollution, managing natural resources and the effects of global warming, applying systemic analyzes to environmental problems.
Environmental sciences became an active field of scientific research in the 1960s and 1970s, due to the need for a multidisciplinary approach to analyzing complex environmental problems, the emergence of various environmental laws requiring specific research protocols, and increasing public awareness. the need for action to solve environmental problems.
RUBISCO!
Ribulose-1,5-bisphosphate carboxylase/oxygenase
