Answer and Explanation:
The steps for construction of the expression plasma
- STEP 1 : Introducing Notl and Hindlll confinement sites at the ends of the gene of interest by means of PCR
- STEP 2 :Digest the plasmid and DNA encoding gene with the Notl and Hindol confinement endonucleuses.
- STEP 3 : Ligate the cut plasmid and DNA fragment
- STEP 4 : Change the plasmid into E.coli for expression
- STEP 5 : Clean the protein by utilizing a column of immobilized
<h2>Answer:</h2>
B.lithosphere is neither destroyed nor produced
<h2>Explanation:</h2>
Transform boundaries are places where plates slide sideways past each other. One plate is forced below another in a process which is called subduction. Many transform boundaries are found on the sea floor, where they connect segments of diverging mid-ocean ridges. In this process, lithosphere is neither created nor destroyed so B is the correct option.
Answer:
Yes
Explanation:
That is what a gaff is used for
The answer is A.) W
The blood is first filtered through the Bowman’s capsule.
Agriculture applies to genetic engineering, not so sure about medicine
