Definitely the best method would be to use immunostaining, <span>this is a protocol that makes sure the use of Triton to break down cell membranes. </span>Immunostaining can be used to see which cells are positive for dynein. It also helps you to m<span>ake sure to counterstain to make cells vissible use hematox when you use light microscopy and DAPI for fluorescence microscopy.</span>