Site-directed mutagenesis is a method in biochemistry/ molecular biology that introduces small changes into the DNA sequence of
a gene. This change alters the amino acid sequence of a protein and can alter the function of a protein. What changes are typically inserted into the DNA sequence? All of the listed statements are true.
deletion Single
base substitution
Short insertion
The mutation is created at a site defined in the DNA sequence. First, it is necessary to synthesize the primer, which contains the mutation. Said primer needs to hybridize with the DNA molecule that has the gene of interest. Using the enzyme DNA polymerase, the mutation is incorporated and allows the formation of the complete DNA. This DNA chain can be introduced into a host cell and subsequently cloned
Explanation:
With this technique it is possible to establish specific mutations, deletions or insertions. Through this it is possible to control the type of mutation that is wanted in the DNA. For example, in the case of deletions, primers are used which, in addition to having their capacity to perform the deletion, should also be able to hybridize the DNA chain. To carry out an insertion, said primer must have the DNA fragment in addition to being able to hybridize the DNA.
Water would move out of the cells of the microorganisms by osmosis and the cytoplasm would become dehydrated, killing the cells. The brine is a hypertonic solution, thereby "pulling" the water out of the bacterial cells.
