Answer:
19.32L
Explanation:
Given parameters:
Volume of the gas at STP = 29.3liters;
Using this information first, let us find the number of moles of the gas.
Number of moles = 
With the number of moles, we can further solve this problem;
Number of moles = 
= 1.31 moles
More parameters;
Pressure on gas = 1.39atm
temperature = -23°C = -23 + 273 = 250K
Unknown:
Volume of gas = ?
Solution:
The ideal gas equation is the perfect fix to solve this problem.
It is mathematically expressed as;
Pv = nRT
where P is the pressure
v is the volume
R is the gas constant
n is the number of moles
T is the temperature
Input the parameters;
1.39 x V = 1.31 x 0.082 x 250
V = 19.32L
Answer:
It yields valuable advice about the product
Explanation:
a company creates value by satisfying customer needs
D. chlorophyll is the main source of light in photosynthesis
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
