How would you correct the mutated cftr protein if you had the ability to tinker with it?

1 answer:

8 0

<span>There several ways to attempt to correct the mutated protein. Gene therapy is possible where a virus vector is used to incorporate a corrected cftr gene into the genome. In the future, the candidate would start producing the necessary correct protein which could reverse symptoms or potentially cure the cystic fibrosis patient. Several side-effects are possible such as extreme reaction to the viral vector or the correct gene could incorporate in the wrong area causing possibly the development of cancer. Other possibilities are CRISPR-CAS9 to precisely edit the genome in the correct spot. The downside is that this makes a permanent alteration in a person's DNA that could be passed on to offspring while the gene therapy would not.</span>

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You experimentally change the DNA sequence directly upstream of a start codon of an operon in E coli to investigate the function

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Answer:

Mutation occurred in ribosome binding site that serves as binding site for 30S ribosomal subunit of <em>E. coli</em> and allows the process of protein synthesis to begin.

Explanation:

The initiation of protein synthesis in bacteria requires binding of the ribosome to the ribosome binding site. The ribosome binding site in bacteria consists of the initiation codon "AUG" and the preceding Shine-Dalgarno sequence. The AUG initiation codon and Shine Delgrano sequence are around 10 bases apart.

The sequence is polypurine hexamer and is represented by 5' ...AGGAGG...3'. Shine-Delgrano sequence is complementary to the conserved sequence present at the 3' end of 16SrRNA of the 30S subunit of the bacterial ribosome. Binding of Shine Delgrano sequence of ribosome binding site and the complementary sequence of the 30S ribosomal subunit marks the first step in the initiation of protein synthesis.

Any mutation in the ribosome binding site would not allow the process of protein synthesis to start or would reduce the rate of the initiation of protein synthesis.

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