Answer:
it is composed of the G1 phase
Explanation:
A) genotype
Because the genotype means the genetic make-up of the cell, so saying that the genes of the plant are homozygous dominant show this genetic make-up of the plant.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The answer is Chordates
Chordates belong to the pylum Chordata of the Animal Kingdom.They have the following characteristics;
Lower vertebrates have a notochord which is a hollow nerve chord that is located dorsally of the animal while higher vertebrates have a vertebral column of bone and cartilage. They have a coelom which is a body cavity that differentiates higher vertebrates into organs and digestive tract.
There are three stages in the life cycle of a spider : - the first is embryonic stage, then the larval stage and finally nympho- imginal stage.
