Answer:
a guide RNA (gRNA or sgRNA) and a CRISPR-associated endonuclease (Cas protein).
Explanation:
The <u>proximal convoluted tubule</u> of the nephron allows the selective reabsorption of useful substances like glucose, amino acids, salts, and water into the blood capillaries.
In the nephron, the proximal convoluted tubule is located between the loop of Henle and Bowman's capsule.
It is the key part of the kidney. The most prominent function of this structure is to reabsorb water, sugar molecules, chloride, and sodium ions from the glomerular filtrate.
The reabsorption of fluids takes place when bulk volumes of sugar and water are transported from PCT to the bloodstream.
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Answer:
4) The mRNA was shorter because of Intron splicing
Explanation:
In eukaryotic cells, the process of gene transcription uses a fragment of DNA as template in order to synthesize a complementary single-stranded RNA molecule known as primary messenger RNA transcript or 'pre-mRNA', which is then processed to yield a mature messenger RNA (mRNA). This mature mRNA is finally used as template to synthesize a polypeptide chain (i.e., a protein) by the process of translation. RNA splicing refers to the processing mechanism by which a pre-mRNA is transformed into a mature mRNA. During RNA splicing, non-coding regions of the pre-mRNA called 'introns' are removed, whereas the remaining coding regions called 'exons' are joined toghether to form a mature mRNA. In consequence, RNA splicing generates an mRNA that is shorter than the originally transcribed gene sequence.
The answer to this question is d