Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
I don't know what your fish looks like but I will try my best :)
To accommodate the developing eggs, the mother's body expands, becoming deeper and broader. A few days before delivery, she develops a bulge below the gills, her outline becoming fairly square in this region, while the gravid spot has enlarged its area. The female swordtail and guppy will both give birth to anywhere from 20 to 100 live young after a gestation period of four to six weeks, and mollies will produce a brood of 20 to 60 live young after a gestation of six to 10 weeks.
Explanation:
Metabolism: all chemical reactions within a cell
Answer: true
Explanation:
Only the mRNA made from the DNA takes part in the protein synthesis
Answer:
The chart shows how well each beetle with each trait were able to survive in their environments, and Mutant 1 was clearly out living the other two, because as you can see mutant 2 has only last for at least one generation, while the non-mutated one lived a little longer. This is because Mutant 1 developed the trait that made its shell darker, and thus it's easier for them to hide from predators, and they were able to successfully pass on their genes to their offspring so now they can do the same.
