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arlik [135]
3 years ago
12

You set up the following cross: Aa:Bb:Cc:Dd x aa:Bb:Cc:dd What proportion of the offspring should have the genotype Aa:bb:CC:dd?

Assume all alleles represented by capital letters are dominant and all genes are independently assorting.

Biology
1 answer:
choli [55]3 years ago
7 0

Answer: The correct answer is 1/64

Explanation:

Given cross is AaBbCcDd x aaBbCcdd

Now, if we consider the cross Aa x aa, the probability of having Aa is 2/4

Similarly, for Bb x Bb, the probability of having bb is 1/4

Cc x Cc, probability of having CC is 1/4

Dd x dd, probability of having dd is 2/4

Thus, the probability or proportion of the offspring to have genotype AabbCCdd = 2/4 x 1/4 x 1/4 x 2/4 = 4/256 = 1/64

Thus, only one out of 64 offspring is expected to have this genotype.

Alternatively, one can draw the Punnett square of AaBbCcDd x aaBbCcdd

Total number of possible genotype combination which can be formed from above cross can be found using the formula 2ⁿ where n is the number of heterozygous pairs.

In AaBbCcDd, there are 4 heterzygous pair and in aaBbCcdd, there are 2 heterozygous pairs.

Thus, n =6

2⁶ = 64

16 type of gametes will be formed by AaBbCcDd and 4 types of gametes will be formed by aaBbCcdd.

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The minimum length of a sgRNA sequence to avoid off target cleavage by the CRISPR/Cas system in the fly fruit genome is 14 bases

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Also, to know how many times a sequence will appear in a genome (the expected value of occurrence), we must multiply the probability of that sequence to randomly occur by the length of the genome. For our specific example, the number of occurence of a sequence of length "n" is:

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But in this case, what we want is the expected number of times the sequence will appear to be 1, and we want to obtain the length of the target sequence (n).

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As the sequence needs to have a natural number of elements, <u>we can conclude that using a target sequence of a minimum of 14 bases with the CRISPR/Cas system in the fly fruit genome should be enough to avoid off target cleavage.</u>

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