Question

As part of your research, you examine mRNA expression for the parents, their two unaffected children, and their affected child with a Northern blot using a full-length cDNA probe.Your results, shown here, are as follows:Both parents have low, but detectable, mRNA expression.The unaffected children have either normal or low mRNA expression.The affected child has no detectable mRNA expression. What possible molecular defect could explain these results?Select every legitimate possibility from the choices below.a. The recessive allele could be a premature stop codon that prevents the translation of a full-length protein.b. The recessive allele could be a mutation in the promoter region that prevents RNA polymerase II from binding.c. The recessive allele could be a mutation in the transcribed region that makes the mRNA unstable and causes it to degrade.d. The recessive allele could be a mutation in regulatory DNA that prevents chromatin from being remodeled from a closed to an open state.e. The recessive allele could be a mutation in a splice donor or acceptor sequence that prevents proper mRNA splicing, leaving an intron in the spliced transcript.

Answer 1

Answer:

b.c d are the answer options

Explanation:

b. The recessive allele could be a mutation in the promoter region that prevents RNA polymerase II from binding.

c. The recessive allele could be a mutation in the transcribed region that makes the mRNA unstable and causes it to degrade.

d. The recessive allele could be a mutation in regulatory DNA that prevents chromatin from being remodeled from a closed to an open state.

in option b, the synthesis of mRNA is going to be halted

in option c, mRNA is undergoing degradation, it will be invisible in the northern blot.

in option d, mRNA in expression of each gene is going to be turned off.

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