Answer:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Explanation:
cDNA is the acronym for complementary DNA. This DNA is a single or double stranded moolecule that is synthesized from a messenger RNA molecule. This molecule is used in studies that aim to clone eukaryotic genes in prokaryotic individuals. The result of this is the expression of a protein from a eukaryotic individual in prokaryotic individuals.
To form a double-stranded cDNA molecule, the following steps would be required:
- DNA to be cloned
- mature mammalian mRNA is isolated
- reverse transcriptase, provided with a primer, synthesizes a strand of cDNA without introns
- the mRNA is hydrolyzed by raising the pH
- a primer site is created on the ssDNA
- a complementary DNA strand is synthesized, forming a ds cDNA molecule.
- dsDNA
Answer:
Option (3)
Explanation:
Unconformity is defined as the gaps in the sequence of geological rocks. It is a geological contact that marks the boundary between the different types of rocks. In simple words, they are also known as the time of non-deposition. These unconformities have great significance in the field of geology. They are of 4 types, namely-
(1) Angular unconformity- The sedimentary rocks are initially formed and deposited in an area, after that the area is tilted and erosion takes place. Now, the new sediments are deposited forming an angle, which is commonly known as an angular unconformity.
(2) Non-conformity- When the sedimentary rocks are deposited over the igneous or metamorphic rocks, then it is known as the non-conformity.
(3) Para conformity- When the sedimentary rocks are deposited parallel to the ground surface, and absence of erosion activity, then these surface appears to be a normal (simple) bedding plane. This is known as the para conformity.
(4) Disconformity- When the sedimentary rocks are formed and undergoes erosion, then new sedimentary rocks are deposited over this and diminishes the unconformity. This forms a discontinuity.
Thus, the unconformity arises due to both erosion and lack of rock deposition.
Hence, the correct answer is option (3).
<span>There must be adequate amounts of NAD available to accept hydrogen atoms.</span>
