Answer:
Essentially there is not an answer. There are two different definitions.
A holoenzyme is an enzyme that needs to be together with a cofactor(s), required for activity.
In some cases, enzymes constituted by several subunits (like DNA polymerase 3) are call holoenzyme as well, because it will only perform correctly when all its subunits are present.
Answer:
The correct answer is - acidic conditions wouldn't trigger a change in the color of Alizarin yellow.
Explanation:
The growth of E. coli generally occurs at neutral pH, however, its growth is normal at acidic conditions as well. The change in the growth of E. coli is not able to detect by alizarin.
The phenol red turns yellow in the presence of an acid, and the change in pH in an alkaline environment can be detected by the red color of phenol red. Growth of E.coli will grow in pH of 10-12 . But, very slowly. The color change in alizarin is also apparent at pH 10.2 to 12 only.
If a star is located 100 light years from earth the light will tack 100 years to reach earth I hope I helped you and good luck