I believe that substance would be water.
The most important differences between a transmission electron microscope and a scanning electron microscope are: Rather than the broad static beam used in TEM, the SEM beam is focused to a fine point and scans line by line over the sample surface in a rectangular raster pattern
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(jacemorris04)
In biology, the biotic factors pertain to the living components of Earth while the abiotic factors are those non-living chemicals and physical factors. The only choice that is not an interaction between biotic and abiotic factors is the third choice, "C. male elk fighting over a female".
Option C:
B. wild-type 5-AUGCAUACAUUGGAGUGA-3 mutant 5'-AUGCAUACAUCUGGAGUGA- 3'
Explanation:
A frameshift mutation is a genetic mutation such that the triplet nature of gene expression by codons, the insertion or deletion occurs in such as way that it alters the complete sequence of 3 codon sets and can change the reading frame, resulting in a completely different translation from the original.
As it is pertinent to understanding that all genes are normally translated in sets of 3 codons, which typically describes and explain a unique amino acid sequence. Thus, if a frame shift mutation occurs, it can affect many amino acid sequences and finally the entire protein.
If we look at option B:
wild-type 5-AUG CAU ACA UUG GAG UGA-3
mutant 5’- AUG CAU ACA UCU GGA GUG A-3’
An insertion of a base Uracil showed at the end of 3rd codon which affected and altered the whole sequence of codon triplets. This changed all next codons and finally all next amino acids will be altered.
A frame shift mutation is the shift of the genetics of an object.
This type of mutation os caused by the insertion or deletion of nucleotides in a DNA sequence that is not divisible by three.