Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
The answer is B. The ocean will absorb more water because the land reflects it back.
Answer:
i think you forgot to pu in the answer choices
Explanation:
Step one: Travel approximately 18 inches up the underside of the branch you are removing. Cut up about halfway through the branch.
Step two: Move to the top side of the branch. Choose a location an inch further out from your first cut. Carefully cut down until the branch breaks free.
Step three: Find the branch collar on your trunk. This is stem tissue around the base of the branch. . Make a complete cut with a 45-degree angle kicking out from the base of the tree.
(SOURCE Chris Lambton) Add your vocabulary words in along the way if you decide to use my answer.
Answer:
The DNA of the baby shares the DNA of both father and mother, and hence a cross link between an O type mother (Taylor) and an AB type father (Tyler) will produce children with blood groups A and B. It will be impossible to give birth to a child with blood group O.
