The answer to this given question is the letter "C". The basis of immunoassay is the unknown amount of drug in a sample competes with the known amount os fluorescently labeled drug for binding to an antibody to that drug.
When one phosphate group is removed by breaking a phosphoanhydride bond in a process called hydrolysis, energy is released, and ATP is converted to adenosine diphosphate (ADP). ... Likewise, energy is also released when a phosphate is removed from ADP to form adenosine monophosphate (AMP).