Atherosclerosis refers to the build-up of fats and cholesterol in the walls of blood vessels (Option A).
<h3>What is atherosclerosis?</h3>
Atherosclerosis is a disease characterized by the build-up of fats and cholesterol in the walls of blood vessels.
This build-up of fats narrows the blood vessels, thereby difficulting the movement of blood in the bloodstream.
The symptoms of atherosclerosis include, among others, chest pain, shortness of breath and fatigue.
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Answer:
First, third, and fifth are the answers. Glucose is broken down into carbon dioxide and water. Light energy is absorbed by chlorophyll. Energy is used to convert carbon dioxide and water into sugar.
Explanation
While the absorbance at 420 nm is related to the amount of o-nitrophenol produced, the absorbance at 600 nm is proportional to cell density, which aids in standardizing our estimations of enzyme activity.
<h3>Describe absorbance.</h3>
The amount of light absorbed by a solution is measured by its absorbance (A), often referred to as optical density (OD). The amount of light that may flow through a solution is called its transmittance.
<h3>How is the activity of beta-galactosidase determined?</h3>
The colorless ONPG substrate is changed by beta-Galactosidase into galactose and the chromophore o-nitrophenol, which results in a vivid yellow solution. The amount of substrate transformed at 420 nm can be calculated by measuring the solution's beta-galactosidase activity using a spectrophotometer or a microplate reader.
<h3>What is measured by the beta-galactosidase assay?</h3>
The -Gal Assay Kit gives users the tools they need to swiftly assess the amounts of active beta-galactosidase expressed in cells that have been transfected with plasmids encoding the lacZ gene.
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Answer:
D. The methyl group of acetyl CoA becomes radio-labeled
Explanation:
During the steps in glycolysis, the carbon at position 1, becomes C-1 in dihydroxyacetone phosphate during the cleavage of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Subsequently on isomerization of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, C-1 of dihydroxyacetone phosphate becomes C-3 of glyceraldehyde-3-phosphate.
Furthermore, in pyruvate, the end product of glycolysis, C-3 is converted to a methyl group which then becomes the methyl group in the acetyl-CoA molecule produced from the oxidative decarboxylation of pyruvate.
Since the radioactive 14-C of radio-labeled glucose occupies position 1, it will become the methyl group of acetyl-CoA.
