The answer is E I believe
Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
<h2>Answer:</h2>
Family of Synthetic Elements
<h2>Explanation:</h2>
In chemical Sciences a synthetic element is a chemical element that does not occur naturally on Earth, and it can only be created artificially in the laboratory. So far, 24 synthetic elements have been created in the laboratory and their atomic numbers lie from 95–118.
Answer:
The correct answer is - all of the above.
Explanation:
Rheumatoid arthritis (RA) causes aggravation of the linning of the joints, particularly in the hands, knee, and fingers. Signs and side effects incorporate red, swollen, excruciating joints, and diminished flexibility and mobility. Articular cartilage is damaged and fibrous tissue invades
If left untreated, it can make extreme harm to the joints and serious complexities in the significant organs. In it, fibrous tissues ossify and bones fuse Rheumatoid joint inflammation is an immune system illness and osteoarthritis is brought about by impacts of maturing. The synovial membrane becomes inflamed and thickens, forming a mass.
The correct answer is - all of the above.
Answer:
long-read sequencing
Explanation:
a method of dna sequencing under active development
