TOPOGRAPHY AND ELEVATION .
Answer:
In order to clone the sequence of the gene for one of the human crystallins found in the eye, there is a need for the application of polymerase chain reaction. In the process, the particular sequence of gene is denatured and then replicated various times to generate various clones of the gene sequence.
By producing various copies of the gene sequence for the human crystallin, that is, desirable, a scientist can examine various distinct characteristics of the protein as there is always more to examine with.
Answer:
Mutation occurred in ribosome binding site that serves as binding site for 30S ribosomal subunit of <em>E. coli</em> and allows the process of protein synthesis to begin.
Explanation:
The initiation of protein synthesis in bacteria requires binding of the ribosome to the ribosome binding site. The ribosome binding site in bacteria consists of the initiation codon "AUG" and the preceding Shine-Dalgarno sequence. The AUG initiation codon and Shine Delgrano sequence are around 10 bases apart.
The sequence is polypurine hexamer and is represented by 5' ...AGGAGG...3'. Shine-Delgrano sequence is complementary to the conserved sequence present at the 3' end of 16SrRNA of the 30S subunit of the bacterial ribosome. Binding of Shine Delgrano sequence of ribosome binding site and the complementary sequence of the 30S ribosomal subunit marks the first step in the initiation of protein synthesis.
Any mutation in the ribosome binding site would not allow the process of protein synthesis to start or would reduce the rate of the initiation of protein synthesis.
The disorder allele is recessive
DNA and RNA have completely different purposes in us. DNA stores and transfers genetic information (Eye and hair colour, skin colour, etc..). RNA codes amino acids and makes proteins. DNA is double stranded while RNA is single stranded. DNA uses adenine, thymine, cytosine, and guanine; while RNA uses adenine, cytosine, uracil, and guanine.
