Question

You are performing a Gram stain on g bacteria and you stop after the addition of the first dye. What is the appearance of the bacteria at this point?

Answer 1

Answer:

The correct answer is violet/ purple.

Explanation:

Gram staining is performed to distinguish between gram-positive and gram-negative bacteria. The first stain or dye which is used in gram stain is crystal violet. So after adding this dye, all cells will be colored purple.

So when we stop after the addition of first dye then all the cells will appear purple/violet because all the cells will contain crystal violet in its cell wall which can be seen under the microscope. So we will not be able to distinguish between gram-positive and gram-negative bacteria. So the correct answer is purple.