Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide
5'-TTTTTTTTTTTTTTTTTTTTTTTTT-3' has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyse the cellular nucleic acids that have stuck to the beads, what will be most abundant?
mRNA because it is the only type of RNA that is polyadenylated and the poly (A) tail would be able to base pair with the strands of poly(T) on the beads and thus stick to them. DNA would not be found in the sample, as the poly (A) tail is not encoded int he DNA and long runs of T are rare in DNA.
The question is incomplete as it does not have the options which are:
(a)DNA (b)tRNA (c)rRNA (d)mRNA
Answer:
Option-mRNA
Explanation:
The mRNA is synthesized from the DNA and which is also a type of nucleic acid. When the nucleic acid is extracted from the yeasts and then mixed with the resin beads to which the polynucleotide with thymine base oar is attached.
The mRNA will bind to the resin bead as the mRNA after synthesis undergoes modification and attaches poly A tail to the end of the mRNA at 3' end.
This poly-A tail has adenine which easily binds to the thymine and thus mRNA easily attaches to the resin bead.
The type of preparation that must be patted onto the skin rather
than rubbed in is lotion. The reason why it should be patted is to prevent increase
circulation and itching. Lotions are water base and for external use only to
soothe or protect the skin from dryness.
