The flow of energy from the Earth's interior originates from two main sources: the radiogenic and primordial heat respectively. Radiogenic heat is produced by the radioactive decay of isotopes in the mantle, whereas primordial heat is the energy left over since the Earth's formation.
Without using any sensitive scientific equipment, this flow of energy is observable at stress points between the tectonic plates, such as volcanoes. These are ruptures in the crust of the planet through which materials from the interior of the planet are expelled.
Baby girls are 4.1mm
Baby boys a 3mm
Nucleotide!
When attached in a polymer, they become a nucleic acid.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
