<span>The most crucial principle of ELISA (enzyme-linked immunosorbent assay) is a highly specific antibody-antigen interaction.</span>
ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in the biological sample. Simply described, in an ELISA, an antigen is immobilized on a solid surface and then a specific antibody is applied over the surface so that it can bind to the antigen. The antibody is usually linked to an enzyme, and in the final step, a substrate for that enzyme is added. The enzyme can convert it to some detectable signal, most commonly a color change. <span>Medical usage of ELISA is in the diagnosis of HIV infection, pregnancy tests, measurement of cytokines…</span>
There are many different factors, but some are: the amount of natural resources (food, water, etc.), amount of predator/prey, and availability of shelter.
Fungi contain many different microscopic organisms compared to regular plants, Fungi can be very poisonous compared to plant too and also a regular plant contains a cell wall where as Fungi don't. Most importantly plants use the process photosynthesis to make their own food, Fungi don't.
The roots need all the water it can get but sense theres water in the ground it already has water which transports water to the leaves