Answer:
One significant difference that I noticed was the color of the water. In the control aquarium, the color of the water at 30 days was the same as it was on the 1st day. In the experimental aquarium, the water had a green tint on the 30th day, but on the first day it was clear.
Transpiration is the process by which moisture is carried through plants from roots to small pores on the underside of leaves, where it changes to vapor and is released to the atmosphere. Transpiration is essentially evaporation of water from plant leaves. Transpiration also includes a process called guttation, which is the loss of water in liquid form from the uninjured leaf or stem of the plant, principally through water stomata.
Studies have revealed that about 10 percent of the moisture found in the atmosphere is released by plants through transpiration. The remaining 90 percent is mainly supplied by evaporation from oceans, seas, and other bodies of water (lakes, rivers, streams).
Transpiration and plant leaves
Plants put down roots into the soil to draw water and nutrients up into the stems and leaves. Some of this water is returned to the air by transpiration (when combined with evaporation, the total process is known as evapotranspiration). Transpiration rates vary widely depending on weather conditions, such as temperature, humidity, sunlight availability and intensity, precipitation, soil type and saturation, wind, land slope, and water use and diversion by people. During dry periods, transpiration can contribute to the loss of moisture in the upper soil zone, which can have an effect on vegetation and food-crop fields.
If a bacterium cannot use citrate agar will not change its color (stays green). On the other hand, if bacteria have the ability to use citrate, the medium will change its color from green to blue.
This happens because citrate agar contains pH indicator such as bromothymol blue which transforms from green to blue in alkaline conditions.